Daily Bulletin

The Conversation

  • Written by Xavier Conlan, Senior Lecturer Forensic Chemistry, Deakin University

The scientists who developed the ability to see some of the building blocks of life under the electron microscope have been awarded the 2017 Nobel Prize in Chemistry.

Jacques Dubochet, Joachim Frank and Richard Henderson pioneered cryo-electron microscopy, which the Royal Swedish Academy of Sciences said both simplifies and improves the imaging of biological molecules, known as biomolecules.

The 9 million Swedish kronor (A$1.4 million) prize is split equally between Dubochet, at Switzerland’s University of Lausanne, Frank, at New York’s Columbia University, and Henderson, at the MRC Laboratory of Molecular Biology, Cambridge in the UK.

image The 2017 winners of the Nobel Prize in Chemistry: (left to right) Joachim Frank, Richard Henderson and Jacques Dubochet. Reuters/Brendan McDermid, Toby Melville and Denis Balibouse

The Academy said the method developed by the three researchers had moved biochemistry into a new era. The technology now allows researchers to generate a high resolution view of biomolecules while they exist in their natural state.

Read more: An award with real gravity: how gravitational waves attracted a Nobel Prize

The biological lock and key

The human body is amazingly complex and requires the cooperation of a range of biochemical mechanisms, such as digestion and energy production, in order to function well. These intricate processes involve the use of biomolecules, typically large entities made from amino acids – the building blocks of life.

Importantly, just like the construction of any brick-built house, the configuration or placement of the blocks is critical to how well our construction stands up, or how well our biomolecules function.

Furthermore, biomolecules present their capacity to perform tasks by interacting with other entities, such enzymes, in the body. These are based on a specific configuration, much like how only one key can open a particular lock.

The significant challenge overcome by the award-winning team was to develop the capacity to observe the biomolecules in their natural state. Before the advent of cryo-electron microscopy, they were visualised with X-Ray crystallography.

It was also thought that electron microscopes were only suitable for imaging dead matter, because the powerful electron beam destroys biological material.

The key breakthrough came with the development of a process to rapidly freeze a sample. This enabled the biomolecules to be captured in their bespoke configuration.

The team identified early on in their work that freezing a sample prior to visualisation may afford the improvement required to fully interrogate the biomolecules.

Frozen in time

Here is where the fun starts. While sounding inherently simple, rapidly freezing a sample is particularly challenging.

If the process removes the water from the sample then the biomolecule collapses, losing the natural configuration desired by the researchers. If the sample is frozen too slowly then ice crystals form, which also interferes with the biomolecule’s configuration.

The team developed a process known as vitrification. This freezes the sample at -190℃ while it is placed on a wire mesh, an elegantly simple approach to solving a difficult problem.

Like most Nobel prizewinning scientific achievements, the development was incremental. Changes by the team over many years enabled the combination of the freezing process (developed in 1978) and the microscopy technology which was only fully realised in 2013.

This combination and advancement in technology enabled the high-resolution imaging of biomolecules.

Unlocking a virus

So what does all this mean? Well, understanding the configuration of the lock enables scientists to cut a particular key.

Viruses are large biomolecules. Once visualised, scientists can identify molecules or develop pharmaceutical keys that can fit into their structure in order to break them apart or disrupt their function.

image Over the past few years, researchers have published atomic structures of numerous complicated protein complexes: (left, a) a protein complex that governs the circadian rhythm, (centre, b) a sensor of the type that reads pressure changes in the ear and allows us to hear, and (right, c) the Zika virus. The Royal Swedish Academy of Sciences

An example of the power of cryo-electron microscopy is seen through the rapid characterisation of the Zika virus soon after it was first identified as a major global health risk.

The identification of the configuration of the virus and the pocket of the biomolecule that joins to its host will form the basis for ongoing studies on how best to combat this virus.

Read more: Error correcting the things that go wrong at the quantum computing scale

The technology has also had an impact at the dinner table. A US research team has investigated the heat-sensing component of the tongue, highlighting the wasabi sensor. This may offer the potential to better understand new pain-management approaches.

In Australia, a consortium is exploiting the power of this technology to probe diseases related to the immune system in order to develop better treatment protocols.

Cryo-electron microscopy will be an exciting area to watch in the near future, for locksmiths and science enthusiasts alike.

Authors: Xavier Conlan, Senior Lecturer Forensic Chemistry, Deakin University

Read more http://theconversation.com/life-frozen-in-time-under-an-electron-microscope-gets-a-nobel-prize-85246

Writers Wanted

Australia's just signed up for a shot at 9 COVID-19 vaccines. Here's what to expect


Road Trip Packing List: Things to Take While Traveling in Australia


The Conversation


Did BLM Really Change the US Police Work?

The Black Lives Matter (BLM) movement has proven that the power of the state rests in the hands of the people it governs. Following the death of 46-year-old black American George Floyd in a case of ...

a Guest Writer - avatar a Guest Writer

Scott Morrison: the right man at the right time

Australia is not at war with another nation or ideology in August 2020 but the nation is in conflict. There are serious threats from China and there are many challenges flowing from the pandemic tha...

Greg Rogers - avatar Greg Rogers

Prime Minister National Cabinet Statement

The National Cabinet met today to discuss Australia’s COVID-19 response, the Victoria outbreak, easing restrictions, helping Australians prepare to go back to work in a COVID-safe environment an...

Scott Morrison - avatar Scott Morrison

Business News

What happens to all those pallets?

Pallets — they're not something everyday people often give much thought to. But they're an integral part of any business which receives or distributes large quantities of goods. But once the goo...

News Company - avatar News Company

Ten tips for landing a freelance transcription job

Transcription jobs are known to be popular in the field of freelancing. They offer fantastic job opportunities to a lot of people, but there are some scammers who wait to cheat the freelancers. ...

News Company - avatar News Company

How To Remove Rubbish More Effectively

It can be a big task to remove household rubbish. The hardest part is finding the best way to get rid of your junk. It can be very overwhelming to know exactly where to start with so many option...

News Company - avatar News Company

News Company Media Core

Content & Technology Connecting Global Audiences

More Information - Less Opinion